primer design for qrt-pcr assay (PrimerDesign Inc)
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Primer Design For Qrt Pcr Assay, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Quantitative RT-PCR:Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus Article Snippet: With very stringent criteria for Article Title: NaCl stress-induced transcriptomics analysis of Salix linearistipularis (syn. Salix mongolica). Article Snippet: Additional file 7: Figure S7. Article Title: LRR1 involved in the abscisic acid signaling pathway to regulate the early growth and development of Arabidopsis thaliana Article Snippet: The primers utilized in this study are listed in ( Article Title: Distribution and accumulation of cassava brown streak viruses within infected cassava (Manihot esculenta) plants Article Snippet: Primer design and determination of virus titre with qRT-PCR For Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus Article Snippet: Very stringent criteria for Article Title: Evaluation of internal control for gene expression in Phalaenopsis by quantitative real-time PCR. Article Snippet: The selection of appropriate reference genes is one of the most important steps to obtain reliable results for normalizing quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) of MADS-box gene in Phalaenopsis.. In this study, we cloned 12 candidate reference genes including 18S ribosomal RNA (18S), elongation factor 1 alpha (EF1α), cytoskeletal structural protein actin (ACT1, ACT2, ACT3, ACT4, ACT5), ubiquitin protein (UBQ1 and UBQ2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the cytoskeletal structural proteins α-tubulin (TUA) and β-tubulin (TUB) in Phalaenopsis and evaluated their expression reliability.. The expression of these candidate reference genes was analyzed using geNorm and normFinder software packages; the results showed that ACT2 and ACT4 were the highest stability reference genes for all experiment sets based on normFinder, followed by ACT1 or ACT3, while ACT3 and ACT4 were the highest stability reference genes for most experiment sets based on geNorm, then TUB or others. Article Title: Characterization of Prolyl Oligopeptidase Genes Differentially Expressed Between Two Cultivars of Coffea arabica L. Article Snippet: Two closely related, Coffea arabica cultivars Tall Mokka and Typica, yield coffee with excellent flavor, but they differ distinctively in the size of aerial organs including the fruit and in the degree of branching.. Using potato cross-species microarray hybridization, we identified prolyl oligopeptidase (CaPOP) gene(s) as differentially expressed between the shoot tips of ‘Tall Mokka’ and ‘Typica’.. Isolation and sequencing of these POP genes identified three paralogs, CaPOP1, CaPOP2 and CaPOP3. Cloning:Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus Article Snippet: With very stringent criteria for Article Title: NaCl stress-induced transcriptomics analysis of Salix linearistipularis (syn. Salix mongolica). Article Snippet: Additional file 7: Figure S7. Article Title: LRR1 involved in the abscisic acid signaling pathway to regulate the early growth and development of Arabidopsis thaliana Article Snippet: The primers utilized in this study are listed in ( Article Title: Distribution and accumulation of cassava brown streak viruses within infected cassava (Manihot esculenta) plants Article Snippet: Primer design and determination of virus titre with qRT-PCR For Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus Article Snippet: Very stringent criteria for Article Title: Evaluation of internal control for gene expression in Phalaenopsis by quantitative real-time PCR. Article Snippet: The selection of appropriate reference genes is one of the most important steps to obtain reliable results for normalizing quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) of MADS-box gene in Phalaenopsis.. In this study, we cloned 12 candidate reference genes including 18S ribosomal RNA (18S), elongation factor 1 alpha (EF1α), cytoskeletal structural protein actin (ACT1, ACT2, ACT3, ACT4, ACT5), ubiquitin protein (UBQ1 and UBQ2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the cytoskeletal structural proteins α-tubulin (TUA) and β-tubulin (TUB) in Phalaenopsis and evaluated their expression reliability.. The expression of these candidate reference genes was analyzed using geNorm and normFinder software packages; the results showed that ACT2 and ACT4 were the highest stability reference genes for all experiment sets based on normFinder, followed by ACT1 or ACT3, while ACT3 and ACT4 were the highest stability reference genes for most experiment sets based on geNorm, then TUB or others. Article Title: Characterization of Prolyl Oligopeptidase Genes Differentially Expressed Between Two Cultivars of Coffea arabica L. Article Snippet: Two closely related, Coffea arabica cultivars Tall Mokka and Typica, yield coffee with excellent flavor, but they differ distinctively in the size of aerial organs including the fruit and in the degree of branching.. Using potato cross-species microarray hybridization, we identified prolyl oligopeptidase (CaPOP) gene(s) as differentially expressed between the shoot tips of ‘Tall Mokka’ and ‘Typica’.. Isolation and sequencing of these POP genes identified three paralogs, CaPOP1, CaPOP2 and CaPOP3. Ubiquitin Proteomics:Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus Article Snippet: With very stringent criteria for Article Title: NaCl stress-induced transcriptomics analysis of Salix linearistipularis (syn. Salix mongolica). Article Snippet: Additional file 7: Figure S7. Article Title: LRR1 involved in the abscisic acid signaling pathway to regulate the early growth and development of Arabidopsis thaliana Article Snippet: The primers utilized in this study are listed in ( Article Title: Distribution and accumulation of cassava brown streak viruses within infected cassava (Manihot esculenta) plants Article Snippet: Primer design and determination of virus titre with qRT-PCR For Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus Article Snippet: Very stringent criteria for Article Title: Evaluation of internal control for gene expression in Phalaenopsis by quantitative real-time PCR. Article Snippet: The selection of appropriate reference genes is one of the most important steps to obtain reliable results for normalizing quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) of MADS-box gene in Phalaenopsis.. In this study, we cloned 12 candidate reference genes including 18S ribosomal RNA (18S), elongation factor 1 alpha (EF1α), cytoskeletal structural protein actin (ACT1, ACT2, ACT3, ACT4, ACT5), ubiquitin protein (UBQ1 and UBQ2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the cytoskeletal structural proteins α-tubulin (TUA) and β-tubulin (TUB) in Phalaenopsis and evaluated their expression reliability.. The expression of these candidate reference genes was analyzed using geNorm and normFinder software packages; the results showed that ACT2 and ACT4 were the highest stability reference genes for all experiment sets based on normFinder, followed by ACT1 or ACT3, while ACT3 and ACT4 were the highest stability reference genes for most experiment sets based on geNorm, then TUB or others. Article Title: Characterization of Prolyl Oligopeptidase Genes Differentially Expressed Between Two Cultivars of Coffea arabica L. Article Snippet: Two closely related, Coffea arabica cultivars Tall Mokka and Typica, yield coffee with excellent flavor, but they differ distinctively in the size of aerial organs including the fruit and in the degree of branching.. Using potato cross-species microarray hybridization, we identified prolyl oligopeptidase (CaPOP) gene(s) as differentially expressed between the shoot tips of ‘Tall Mokka’ and ‘Typica’.. Isolation and sequencing of these POP genes identified three paralogs, CaPOP1, CaPOP2 and CaPOP3. Virus:Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus Article Snippet: With very stringent criteria for Article Title: NaCl stress-induced transcriptomics analysis of Salix linearistipularis (syn. Salix mongolica). Article Snippet: Additional file 7: Figure S7. Article Title: LRR1 involved in the abscisic acid signaling pathway to regulate the early growth and development of Arabidopsis thaliana Article Snippet: The primers utilized in this study are listed in ( Article Title: Distribution and accumulation of cassava brown streak viruses within infected cassava (Manihot esculenta) plants Article Snippet: Primer design and determination of virus titre with qRT-PCR For Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus Article Snippet: Very stringent criteria for Article Title: Evaluation of internal control for gene expression in Phalaenopsis by quantitative real-time PCR. Article Snippet: The selection of appropriate reference genes is one of the most important steps to obtain reliable results for normalizing quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) of MADS-box gene in Phalaenopsis.. In this study, we cloned 12 candidate reference genes including 18S ribosomal RNA (18S), elongation factor 1 alpha (EF1α), cytoskeletal structural protein actin (ACT1, ACT2, ACT3, ACT4, ACT5), ubiquitin protein (UBQ1 and UBQ2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the cytoskeletal structural proteins α-tubulin (TUA) and β-tubulin (TUB) in Phalaenopsis and evaluated their expression reliability.. The expression of these candidate reference genes was analyzed using geNorm and normFinder software packages; the results showed that ACT2 and ACT4 were the highest stability reference genes for all experiment sets based on normFinder, followed by ACT1 or ACT3, while ACT3 and ACT4 were the highest stability reference genes for most experiment sets based on geNorm, then TUB or others. Article Title: Characterization of Prolyl Oligopeptidase Genes Differentially Expressed Between Two Cultivars of Coffea arabica L. Article Snippet: Two closely related, Coffea arabica cultivars Tall Mokka and Typica, yield coffee with excellent flavor, but they differ distinctively in the size of aerial organs including the fruit and in the degree of branching.. Using potato cross-species microarray hybridization, we identified prolyl oligopeptidase (CaPOP) gene(s) as differentially expressed between the shoot tips of ‘Tall Mokka’ and ‘Typica’.. Isolation and sequencing of these POP genes identified three paralogs, CaPOP1, CaPOP2 and CaPOP3. Gene Expression:Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus Article Snippet: With very stringent criteria for Article Title: NaCl stress-induced transcriptomics analysis of Salix linearistipularis (syn. Salix mongolica). Article Snippet: Additional file 7: Figure S7. Article Title: LRR1 involved in the abscisic acid signaling pathway to regulate the early growth and development of Arabidopsis thaliana Article Snippet: The primers utilized in this study are listed in ( Article Title: Distribution and accumulation of cassava brown streak viruses within infected cassava (Manihot esculenta) plants Article Snippet: Primer design and determination of virus titre with qRT-PCR For Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus Article Snippet: Very stringent criteria for Article Title: Evaluation of internal control for gene expression in Phalaenopsis by quantitative real-time PCR. Article Snippet: The selection of appropriate reference genes is one of the most important steps to obtain reliable results for normalizing quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) of MADS-box gene in Phalaenopsis.. In this study, we cloned 12 candidate reference genes including 18S ribosomal RNA (18S), elongation factor 1 alpha (EF1α), cytoskeletal structural protein actin (ACT1, ACT2, ACT3, ACT4, ACT5), ubiquitin protein (UBQ1 and UBQ2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the cytoskeletal structural proteins α-tubulin (TUA) and β-tubulin (TUB) in Phalaenopsis and evaluated their expression reliability.. The expression of these candidate reference genes was analyzed using geNorm and normFinder software packages; the results showed that ACT2 and ACT4 were the highest stability reference genes for all experiment sets based on normFinder, followed by ACT1 or ACT3, while ACT3 and ACT4 were the highest stability reference genes for most experiment sets based on geNorm, then TUB or others. Article Title: Characterization of Prolyl Oligopeptidase Genes Differentially Expressed Between Two Cultivars of Coffea arabica L. Article Snippet: Two closely related, Coffea arabica cultivars Tall Mokka and Typica, yield coffee with excellent flavor, but they differ distinctively in the size of aerial organs including the fruit and in the degree of branching.. Using potato cross-species microarray hybridization, we identified prolyl oligopeptidase (CaPOP) gene(s) as differentially expressed between the shoot tips of ‘Tall Mokka’ and ‘Typica’.. Isolation and sequencing of these POP genes identified three paralogs, CaPOP1, CaPOP2 and CaPOP3. |
