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PrimerDesign Inc primer design for qrt-pcr assay
Primer Design For Qrt Pcr Assay, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qrt-pcr+primer+design/qrt+pcr+primer+design/pmc11944418-179-15-19
Average 90 stars, based on 1 article reviews
primer design for qrt-pcr assay - by Bioz Stars, 2026-09
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Related Articles

Quantitative RT-PCR:

Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus
Article Snippet: With very stringent criteria for qRT-PCR primer design ( SI Appendix , SI Materials and Methods ), we found that transcript levels of the six other SDRs remained unchanged in EpSDR-5 –silenced leaf and stem tissue ( SI Appendix , Fig. S11 E and F ).

Article Title: NaCl stress-induced transcriptomics analysis of Salix linearistipularis (syn. Salix mongolica).
Article Snippet: Additional file 7: Figure S7. qRT-PCR Primer design.

Article Title: LRR1 involved in the abscisic acid signaling pathway to regulate the early growth and development of Arabidopsis thaliana
Article Snippet: The primers utilized in this study are listed in (qRT-PCR primer design: through www.ncbi.nlm.nih.gov/tools/primer-blast/ ).

Article Title: Distribution and accumulation of cassava brown streak viruses within infected cassava (Manihot esculenta) plants
Article Snippet: Primer design and determination of virus titre with qRT-PCR For qRT-PCR primer design, 12 complete CBSV and UCBSV sequences available in GenBank (accession numbers FN433932.1, FN433931.1, FN434109.1, FJ039520.1, NC014791.1, HM181930.1, FJ185044.1 for UCBSV; and GQ329864.1, NC012698.2, FN434437.1, FN434436.1, GU563327.1 for CBSV) were aligned using MEGA5 (Tamura et al., 2011).

Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus
Article Snippet: Very stringent criteria for qRT-PCR primer design ( SI Appendix , SI Materials and Methods ) revealed that transcript levels of all other CYP71D clade members in CYP71D365 -silenced leaf and stem tissue were unchanged ( SI Appendix , Fig. S11 C and D ).

Article Title: Evaluation of internal control for gene expression in Phalaenopsis by quantitative real-time PCR.
Article Snippet: The selection of appropriate reference genes is one of the most important steps to obtain reliable results for normalizing quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) of MADS-box gene in Phalaenopsis.. In this study, we cloned 12 candidate reference genes including 18S ribosomal RNA (18S), elongation factor 1 alpha (EF1α), cytoskeletal structural protein actin (ACT1, ACT2, ACT3, ACT4, ACT5), ubiquitin protein (UBQ1 and UBQ2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the cytoskeletal structural proteins α-tubulin (TUA) and β-tubulin (TUB) in Phalaenopsis and evaluated their expression reliability.. The expression of these candidate reference genes was analyzed using geNorm and normFinder software packages; the results showed that ACT2 and ACT4 were the highest stability reference genes for all experiment sets based on normFinder, followed by ACT1 or ACT3, while ACT3 and ACT4 were the highest stability reference genes for most experiment sets based on geNorm, then TUB or others.

Article Title: Characterization of Prolyl Oligopeptidase Genes Differentially Expressed Between Two Cultivars of Coffea arabica L.
Article Snippet: Two closely related, Coffea arabica cultivars Tall Mokka and Typica, yield coffee with excellent flavor, but they differ distinctively in the size of aerial organs including the fruit and in the degree of branching.. Using potato cross-species microarray hybridization, we identified prolyl oligopeptidase (CaPOP) gene(s) as differentially expressed between the shoot tips of ‘Tall Mokka’ and ‘Typica’.. Isolation and sequencing of these POP genes identified three paralogs, CaPOP1, CaPOP2 and CaPOP3.

Cloning:

Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus
Article Snippet: With very stringent criteria for qRT-PCR primer design ( SI Appendix , SI Materials and Methods ), we found that transcript levels of the six other SDRs remained unchanged in EpSDR-5 –silenced leaf and stem tissue ( SI Appendix , Fig. S11 E and F ).

Article Title: NaCl stress-induced transcriptomics analysis of Salix linearistipularis (syn. Salix mongolica).
Article Snippet: Additional file 7: Figure S7. qRT-PCR Primer design.

Article Title: LRR1 involved in the abscisic acid signaling pathway to regulate the early growth and development of Arabidopsis thaliana
Article Snippet: The primers utilized in this study are listed in (qRT-PCR primer design: through www.ncbi.nlm.nih.gov/tools/primer-blast/ ).

Article Title: Distribution and accumulation of cassava brown streak viruses within infected cassava (Manihot esculenta) plants
Article Snippet: Primer design and determination of virus titre with qRT-PCR For qRT-PCR primer design, 12 complete CBSV and UCBSV sequences available in GenBank (accession numbers FN433932.1, FN433931.1, FN434109.1, FJ039520.1, NC014791.1, HM181930.1, FJ185044.1 for UCBSV; and GQ329864.1, NC012698.2, FN434437.1, FN434436.1, GU563327.1 for CBSV) were aligned using MEGA5 (Tamura et al., 2011).

Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus
Article Snippet: Very stringent criteria for qRT-PCR primer design ( SI Appendix , SI Materials and Methods ) revealed that transcript levels of all other CYP71D clade members in CYP71D365 -silenced leaf and stem tissue were unchanged ( SI Appendix , Fig. S11 C and D ).

Article Title: Evaluation of internal control for gene expression in Phalaenopsis by quantitative real-time PCR.
Article Snippet: The selection of appropriate reference genes is one of the most important steps to obtain reliable results for normalizing quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) of MADS-box gene in Phalaenopsis.. In this study, we cloned 12 candidate reference genes including 18S ribosomal RNA (18S), elongation factor 1 alpha (EF1α), cytoskeletal structural protein actin (ACT1, ACT2, ACT3, ACT4, ACT5), ubiquitin protein (UBQ1 and UBQ2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the cytoskeletal structural proteins α-tubulin (TUA) and β-tubulin (TUB) in Phalaenopsis and evaluated their expression reliability.. The expression of these candidate reference genes was analyzed using geNorm and normFinder software packages; the results showed that ACT2 and ACT4 were the highest stability reference genes for all experiment sets based on normFinder, followed by ACT1 or ACT3, while ACT3 and ACT4 were the highest stability reference genes for most experiment sets based on geNorm, then TUB or others.

Article Title: Characterization of Prolyl Oligopeptidase Genes Differentially Expressed Between Two Cultivars of Coffea arabica L.
Article Snippet: Two closely related, Coffea arabica cultivars Tall Mokka and Typica, yield coffee with excellent flavor, but they differ distinctively in the size of aerial organs including the fruit and in the degree of branching.. Using potato cross-species microarray hybridization, we identified prolyl oligopeptidase (CaPOP) gene(s) as differentially expressed between the shoot tips of ‘Tall Mokka’ and ‘Typica’.. Isolation and sequencing of these POP genes identified three paralogs, CaPOP1, CaPOP2 and CaPOP3.

Ubiquitin Proteomics:

Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus
Article Snippet: With very stringent criteria for qRT-PCR primer design ( SI Appendix , SI Materials and Methods ), we found that transcript levels of the six other SDRs remained unchanged in EpSDR-5 –silenced leaf and stem tissue ( SI Appendix , Fig. S11 E and F ).

Article Title: NaCl stress-induced transcriptomics analysis of Salix linearistipularis (syn. Salix mongolica).
Article Snippet: Additional file 7: Figure S7. qRT-PCR Primer design.

Article Title: LRR1 involved in the abscisic acid signaling pathway to regulate the early growth and development of Arabidopsis thaliana
Article Snippet: The primers utilized in this study are listed in (qRT-PCR primer design: through www.ncbi.nlm.nih.gov/tools/primer-blast/ ).

Article Title: Distribution and accumulation of cassava brown streak viruses within infected cassava (Manihot esculenta) plants
Article Snippet: Primer design and determination of virus titre with qRT-PCR For qRT-PCR primer design, 12 complete CBSV and UCBSV sequences available in GenBank (accession numbers FN433932.1, FN433931.1, FN434109.1, FJ039520.1, NC014791.1, HM181930.1, FJ185044.1 for UCBSV; and GQ329864.1, NC012698.2, FN434437.1, FN434436.1, GU563327.1 for CBSV) were aligned using MEGA5 (Tamura et al., 2011).

Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus
Article Snippet: Very stringent criteria for qRT-PCR primer design ( SI Appendix , SI Materials and Methods ) revealed that transcript levels of all other CYP71D clade members in CYP71D365 -silenced leaf and stem tissue were unchanged ( SI Appendix , Fig. S11 C and D ).

Article Title: Evaluation of internal control for gene expression in Phalaenopsis by quantitative real-time PCR.
Article Snippet: The selection of appropriate reference genes is one of the most important steps to obtain reliable results for normalizing quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) of MADS-box gene in Phalaenopsis.. In this study, we cloned 12 candidate reference genes including 18S ribosomal RNA (18S), elongation factor 1 alpha (EF1α), cytoskeletal structural protein actin (ACT1, ACT2, ACT3, ACT4, ACT5), ubiquitin protein (UBQ1 and UBQ2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the cytoskeletal structural proteins α-tubulin (TUA) and β-tubulin (TUB) in Phalaenopsis and evaluated their expression reliability.. The expression of these candidate reference genes was analyzed using geNorm and normFinder software packages; the results showed that ACT2 and ACT4 were the highest stability reference genes for all experiment sets based on normFinder, followed by ACT1 or ACT3, while ACT3 and ACT4 were the highest stability reference genes for most experiment sets based on geNorm, then TUB or others.

Article Title: Characterization of Prolyl Oligopeptidase Genes Differentially Expressed Between Two Cultivars of Coffea arabica L.
Article Snippet: Two closely related, Coffea arabica cultivars Tall Mokka and Typica, yield coffee with excellent flavor, but they differ distinctively in the size of aerial organs including the fruit and in the degree of branching.. Using potato cross-species microarray hybridization, we identified prolyl oligopeptidase (CaPOP) gene(s) as differentially expressed between the shoot tips of ‘Tall Mokka’ and ‘Typica’.. Isolation and sequencing of these POP genes identified three paralogs, CaPOP1, CaPOP2 and CaPOP3.

Virus:

Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus
Article Snippet: With very stringent criteria for qRT-PCR primer design ( SI Appendix , SI Materials and Methods ), we found that transcript levels of the six other SDRs remained unchanged in EpSDR-5 –silenced leaf and stem tissue ( SI Appendix , Fig. S11 E and F ).

Article Title: NaCl stress-induced transcriptomics analysis of Salix linearistipularis (syn. Salix mongolica).
Article Snippet: Additional file 7: Figure S7. qRT-PCR Primer design.

Article Title: LRR1 involved in the abscisic acid signaling pathway to regulate the early growth and development of Arabidopsis thaliana
Article Snippet: The primers utilized in this study are listed in (qRT-PCR primer design: through www.ncbi.nlm.nih.gov/tools/primer-blast/ ).

Article Title: Distribution and accumulation of cassava brown streak viruses within infected cassava (Manihot esculenta) plants
Article Snippet: Primer design and determination of virus titre with qRT-PCR For qRT-PCR primer design, 12 complete CBSV and UCBSV sequences available in GenBank (accession numbers FN433932.1, FN433931.1, FN434109.1, FJ039520.1, NC014791.1, HM181930.1, FJ185044.1 for UCBSV; and GQ329864.1, NC012698.2, FN434437.1, FN434436.1, GU563327.1 for CBSV) were aligned using MEGA5 (Tamura et al., 2011).

Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus
Article Snippet: Very stringent criteria for qRT-PCR primer design ( SI Appendix , SI Materials and Methods ) revealed that transcript levels of all other CYP71D clade members in CYP71D365 -silenced leaf and stem tissue were unchanged ( SI Appendix , Fig. S11 C and D ).

Article Title: Evaluation of internal control for gene expression in Phalaenopsis by quantitative real-time PCR.
Article Snippet: The selection of appropriate reference genes is one of the most important steps to obtain reliable results for normalizing quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) of MADS-box gene in Phalaenopsis.. In this study, we cloned 12 candidate reference genes including 18S ribosomal RNA (18S), elongation factor 1 alpha (EF1α), cytoskeletal structural protein actin (ACT1, ACT2, ACT3, ACT4, ACT5), ubiquitin protein (UBQ1 and UBQ2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the cytoskeletal structural proteins α-tubulin (TUA) and β-tubulin (TUB) in Phalaenopsis and evaluated their expression reliability.. The expression of these candidate reference genes was analyzed using geNorm and normFinder software packages; the results showed that ACT2 and ACT4 were the highest stability reference genes for all experiment sets based on normFinder, followed by ACT1 or ACT3, while ACT3 and ACT4 were the highest stability reference genes for most experiment sets based on geNorm, then TUB or others.

Article Title: Characterization of Prolyl Oligopeptidase Genes Differentially Expressed Between Two Cultivars of Coffea arabica L.
Article Snippet: Two closely related, Coffea arabica cultivars Tall Mokka and Typica, yield coffee with excellent flavor, but they differ distinctively in the size of aerial organs including the fruit and in the degree of branching.. Using potato cross-species microarray hybridization, we identified prolyl oligopeptidase (CaPOP) gene(s) as differentially expressed between the shoot tips of ‘Tall Mokka’ and ‘Typica’.. Isolation and sequencing of these POP genes identified three paralogs, CaPOP1, CaPOP2 and CaPOP3.

Gene Expression:

Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus
Article Snippet: With very stringent criteria for qRT-PCR primer design ( SI Appendix , SI Materials and Methods ), we found that transcript levels of the six other SDRs remained unchanged in EpSDR-5 –silenced leaf and stem tissue ( SI Appendix , Fig. S11 E and F ).

Article Title: NaCl stress-induced transcriptomics analysis of Salix linearistipularis (syn. Salix mongolica).
Article Snippet: Additional file 7: Figure S7. qRT-PCR Primer design.

Article Title: LRR1 involved in the abscisic acid signaling pathway to regulate the early growth and development of Arabidopsis thaliana
Article Snippet: The primers utilized in this study are listed in (qRT-PCR primer design: through www.ncbi.nlm.nih.gov/tools/primer-blast/ ).

Article Title: Distribution and accumulation of cassava brown streak viruses within infected cassava (Manihot esculenta) plants
Article Snippet: Primer design and determination of virus titre with qRT-PCR For qRT-PCR primer design, 12 complete CBSV and UCBSV sequences available in GenBank (accession numbers FN433932.1, FN433931.1, FN434109.1, FJ039520.1, NC014791.1, HM181930.1, FJ185044.1 for UCBSV; and GQ329864.1, NC012698.2, FN434437.1, FN434436.1, GU563327.1 for CBSV) were aligned using MEGA5 (Tamura et al., 2011).

Article Title: Gene discovery and virus-induced gene silencing reveal branched pathways to major classes of bioactive diterpenoids in Euphorbia peplus
Article Snippet: Very stringent criteria for qRT-PCR primer design ( SI Appendix , SI Materials and Methods ) revealed that transcript levels of all other CYP71D clade members in CYP71D365 -silenced leaf and stem tissue were unchanged ( SI Appendix , Fig. S11 C and D ).

Article Title: Evaluation of internal control for gene expression in Phalaenopsis by quantitative real-time PCR.
Article Snippet: The selection of appropriate reference genes is one of the most important steps to obtain reliable results for normalizing quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) of MADS-box gene in Phalaenopsis.. In this study, we cloned 12 candidate reference genes including 18S ribosomal RNA (18S), elongation factor 1 alpha (EF1α), cytoskeletal structural protein actin (ACT1, ACT2, ACT3, ACT4, ACT5), ubiquitin protein (UBQ1 and UBQ2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the cytoskeletal structural proteins α-tubulin (TUA) and β-tubulin (TUB) in Phalaenopsis and evaluated their expression reliability.. The expression of these candidate reference genes was analyzed using geNorm and normFinder software packages; the results showed that ACT2 and ACT4 were the highest stability reference genes for all experiment sets based on normFinder, followed by ACT1 or ACT3, while ACT3 and ACT4 were the highest stability reference genes for most experiment sets based on geNorm, then TUB or others.

Article Title: Characterization of Prolyl Oligopeptidase Genes Differentially Expressed Between Two Cultivars of Coffea arabica L.
Article Snippet: Two closely related, Coffea arabica cultivars Tall Mokka and Typica, yield coffee with excellent flavor, but they differ distinctively in the size of aerial organs including the fruit and in the degree of branching.. Using potato cross-species microarray hybridization, we identified prolyl oligopeptidase (CaPOP) gene(s) as differentially expressed between the shoot tips of ‘Tall Mokka’ and ‘Typica’.. Isolation and sequencing of these POP genes identified three paralogs, CaPOP1, CaPOP2 and CaPOP3.



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Validation by quantitative qRT-PCR . The figure presents a summary of the 10 genes selected for validation by qRT-PCR. Above each gene name are two

Journal: BMC Genomics

Article Title: A Marfan syndrome gene expression phenotype in cultured skin fibroblasts

doi: 10.1186/1471-2164-8-319

Figure Lengend Snippet: Validation by quantitative qRT-PCR . The figure presents a summary of the 10 genes selected for validation by qRT-PCR. Above each gene name are two "box-and-whisker" plots of expression levels for that gene across 32 unaffected control (UC) samples (left plot of each pair, blue, up-triangles) and 42 MFS affected samples (right, red, down-triangles). Vertical axis is log 10 ratio of expression level to the median UC level. Each "box" shows the inter-quartile range (IQR), i.e., the range between the 25 th and 75 th percentiles of the log ratios; the horizontal line in each box is the 50th percentile (median). (Median log ratio for UC is always zero, by definition.) "Whiskers" (vertical lines) extend from each box to the most extreme values within 1.5 times the IQR from the box; in normally distributed data this would on average encompass 99% of the values. Triangles mark more extreme points. The lower curve shows log 10 (p-value) for a Wilcoxon rank sum test of the null hypothesis that the UC and MFS distribution are identical; horizontal line marks the p = 0.05 significance level. 6 of 10 genes have p-values < 0.05 by this test. Most genes exhibit noticeably greater variability across the MFS samples than across UC samples, although the Wilcoxon test is not sensitive to this. To highlight one example, for Elastin ( ELN ), the middle 50% of the UC sample log ratios fall between -0.23 and +0.14 (i.e., the 25 th and 75 th percentiles of the values fall 1.70-fold below and 1.38-fold above the median, respectively), and all but 4 fall between -0.58 and +0.34 (4-fold below and 2.2-fold above median). In contrast, median Elastin level is 26 fold lower in MFS samples, only four MFS samples are above the UC median, and the null hypothesis has a p-value of 1.6 × 10 -8 .

Article Snippet: Validation of the array results was done using Applied Biosystems pre-designed qRT-PCR primer and probe sets (PDAR's, see ABI P/N 4333458_a.pdf for the complete protocol).

Techniques: Quantitative RT-PCR, Whisker Assay, Expressing

 QRT-PCR  validation and phenotype prediction

Journal: BMC Genomics

Article Title: A Marfan syndrome gene expression phenotype in cultured skin fibroblasts

doi: 10.1186/1471-2164-8-319

Figure Lengend Snippet: QRT-PCR validation and phenotype prediction

Article Snippet: Validation of the array results was done using Applied Biosystems pre-designed qRT-PCR primer and probe sets (PDAR's, see ABI P/N 4333458_a.pdf for the complete protocol).

Techniques: